Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 19, Issue -, Pages 649-660Publisher
ELSEVIER
DOI: 10.1016/j.jff.2015.09.064
Keywords
Elderberry fruits; Antioxidant capacity; In vitro digestion; Transepithelial transport; Intracellular ROS generation; Proinflammatory genes expression
Categories
Funding
- Ministry of Science and Higher Education, Poland [N N312211338]
Ask authors/readers for more resources
In order to evaluate potential therapeutic properties of Sambucus nigra fruit extract, its anti-inflammatory effects were investigated in lipopolysaccharide-activated RAW264.7 macrophages. Physiochemical changes in the extract composition, which occur following oral administration, were mimicked in an artificial alimentary tract, while transepithelial absorption was simulated using enterocyte-like Caco-2 cell monolayer, imitating intestinal barrier. The obtained results showed that gastrointestinal digested extract, transported across intestinal epithelium, down-regulated the expression of major genes of inflammatory pathway, such as IL-1 beta, IL-6, TNF-alpha and COX-2. Reduction of IL-6, TNF-alpha and prostaglandin E-2 secretion was also observed as a result of LPS-stimulated macrophages' treatment. Moreover, enhanced nitric oxide production in response to inflammatory stimuli in RAW264.7 cells was controlled by the digested and intestinal permeable fraction of elderberry extract. (C) 2015 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available