Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 18, Issue -, Pages 1125-1137Publisher
ELSEVIER
DOI: 10.1016/j.jff.2015.01.021
Keywords
Date seed; Protein hydrolysate; Antioxidant activity; ACE inhibitory activity
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
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Date seed flour was used to produce protein hydrolysates using Alcalase (AL), Flavourzyme (FL) and Thermolysin (TH). Enzymes were used individually or in combination at a constant pH and temperature. The degree of hydrolysis ranged between 11 and 14%. The molecular weights of peptides determined, using a quadrupole orthogonal time-of-flight hybrid tandem mass spectrometer, ranged from 2062.9 +/- 0.0 to 116,803.8 +/- 0.4 Da. AL hydrolysate showed the lowest reducing power and ABTS radical scavenging activity, but a higher ACE inhibition and hydroxyl radical scavenging. Among all treatments, hydrolysates prepared using a combination of AL and FL exhibited the highest reducing power and metal chelation and high scavenging for ABTS, DPPH, hydroxyl radicals as well as ACE inhibitory activity. Combinations of AL and TH exhibited the highest ACE inhibitory activity with an IC50 value of 0.53 mg/mL. These results indicate that date seed protein hydrolysate could be used as a potential health promoting ingredient with antioxidant and ACE inhibitory activities. (C) 2015 Elsevier Ltd. All rights reserved.
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