Journal
POULTRY SCIENCE
Volume 92, Issue 7, Pages 1758-1763Publisher
POULTRY SCIENCE ASSOC INC
DOI: 10.3382/ps.2012-02998
Keywords
Eimeria tenella; pathogen; immunity; enzyme-linked immunosorbent spot; chicken
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Funding
- National Natural Science Foundation of China [30871862]
- National High Technology Research and Development Program of China [2011AA10A209]
- Yangtze River Scholar and Innovation Research Team Development Program [IRT0945]
- Research and Innovation Project for College Graduates of China Agricultural University [KYCX2011052]
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The enzyme-linked immunosorbent spot (ELISPOT) assay is a sensitive and easy-to-use tool to quantify the number of interferon (IFN)-gamma-producing cells and offers a viable alternative for the quantitative measurement of T cell functions in chickens. To study the development of cell-mediated immunity in Eimeria-infected chickens, we measured the number of IFN-gamma-producing cells in peripheral blood mononuclear cells by ELISPOT after 3 oral inoculations of Eimeria tenella oocysts at 2-wk intervals. We found that the number of IFN-gamma-producing cells was significantly increased at 2 wk after the primary infection compared with the control group. The IFN-gamma-producing cells were further increased after repeated infections, and there was a statistically significant increase in the number of IFN-gamma-producing cells after the third infection than after the first infection. Our results indicated that the ELISPOT assay can be used to quantitatively measure antigen-specific T cell responses to coccidia or other avian pathogens.
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