Evaluation of In-Vitro Cytotoxicity of Composite Materials Composed of Poly-L-lactic Acid and beta-Tricalcium Phosphate

Composite material film composed of Poly-L-lactic acid (PLLA) and beta-tricalcium phosphate (beta-TCP) was prepared by a solvent evaporation technique. Cellular cultivation in vitro, Von Kossa staining and MTT assay were performed. The results of cytotoxicity test show that cells cultured in extracts of PLLA/beta-TCP and on the surface of composite showed normal growth and proliferation, mineralization nodules were observed for fibroblasts cultured in PLLA/beta-TCP extract at day 7. Compared with pure PLLA materials, beta-TCP in the PLLA composite facilitate both adhesion and proliferation of rat fibroblasts on the PLLA/beta-TCP composite film.