Journal
BIOANALYSIS
Volume 7, Issue 6, Pages 763-779Publisher
FUTURE SCI LTD
DOI: 10.4155/bio.14.308
Keywords
-
Funding
- Dutch Technology Foundation (STW) [11056]
- PRA Health Sciences (Assen, the Netherlands)
- Spark Holland (Emmen, the Netherlands)
- Merck Sharp & Dohme (MSD, Oss, the Netherlands)
- TOSOH Bioscience (Stuttgart, Germany)
- Beckman Coulter (Indianapolis, IN, USA)
- Bionavis (Tampere, Finland)
Ask authors/readers for more resources
Antibody-free approaches for quantitative LC-MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature peptides by fractionated protein precipitation; enrichment of target proteins and signature peptides by reversed-phase and ion-exchange solid-phase extraction; and enrichment of target proteins and signature peptides by (antibody-free) affinity-solid-phase extraction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available