Journal
POLYMER JOURNAL
Volume 43, Issue 8, Pages 718-722Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/pj.2011.49
Keywords
alkyl methacrylate; amphiphilic; copolymer; endoplasmic reticulum; fluorescently labeled copolymers; specific organelles
Categories
Funding
- New Energy and Industrial Technology Development Organization
- Grants-in-Aid for Scientific Research [23300176, 23651232] Funding Source: KAKEN
Ask authors/readers for more resources
In this study, a novel polymer material was introduced into cells and was delivered to specific organelles. Various fluorescently labeled copolymers of 2-methacryloyloxyethylphosphorylcholine (MPC) and alkyl methacrylates, such as n-hexyl methacrylate (HMA), n-dodecyl methacrylate (DMA) and stearyl methacrylate (SMA), were synthesized. HeLa cells were treated with the copolymers and subsequently observed by confocal fluorescence microscopy. The results indicated that the localization behavior of the copolymers was dependent on their structure. For instance, poly(HMA-co-MPC) was non-cytotoxic and was localized in specific cellular organelles such as the endoplasmic reticulum. In contrast, poly(DMA-co-MPC) was cytotoxic and was partially transported to specific organelles. Poly(SMA-co-MPC) hardly entered the cells. The mechanism of delivery and structure-function relationships of the copolymers are also discussed. Polymer Journal (2011) 43, 718-722; doi:10.1038/pj.2011.49; published online 15 June 2011
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available