Journal
POLYMER DEGRADATION AND STABILITY
Volume 95, Issue 2, Pages 254-259Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.polymdegradstab.2009.11.001
Keywords
Sterilization; Thermo-gelation; Xyloglucan; Degradation
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Funding
- Agence Nationale de la Recherche [ANR05PRIB01601]
- IngeCELL - Medicen Paris Region
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In this study, the influence of different sterilization methods on the thermo-gelation and structural properties of xyloglucan hydrogels was investigated. Xyloglucan samples were treated by either 70% ethanol, 70% isopropanol, gamma-irradiation (10 kGy) at room temperature, gamma-irradiation (10 kGy) in dry ice or autoclaving. These samples were tested for sterility by incubation with sterile Lysogeny Broth (LB) at room temperature, 30 degrees C and 37 degrees C for 30 days. According to the results obtained, xyloglucan hydrogels were only effectively sterilized by autoclaving or by gamma-irradiation either at room temperature or in dry ice. These samples were analyzed by theology measurements and dynamic and static light scattering analysis. Gamma-irradiation at room temperature markedly changed the polymer structure, preventing thermo-gelation. only autoclaving and gamma-irradiation in dry ice preserved the theological properties of the polymer. The sol-gel transition as a function of the temperature was similar for these samples and the control sample. (C) 2009 Elsevier Ltd. All rights reserved.
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