Journal
POLYMER CHEMISTRY
Volume 5, Issue 3, Pages 1082-1090Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3py00869j
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Funding
- National Basic Research Program of China (973 Program) [2011CB606204, 2012CB619100]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20110172120002]
- National Natural Science Foundation of China [51172073, 51072055, 51202069]
- Program for New Century Excellent Talents in University [NCET-11-0148]
- Fundamental Research Funds for the Central Universities [2012ZP0001]
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The application of enzymatic crosslinking and Diets-Alder (DA) click chemistry for hydrogel formation has recently bloomed, because of the efficient chemical bonding and the mild biological reaction conditions. In this study, an injectable hyaluronic acid/PEG (HA/PEG) hydrogel was successfully fabricated for the first time through integrating two cross-linking processes, including firstly enzymatic crosslinking and subsequent DA click chemistry. The enzymatic crosslinking resulted in a fast gelation of HA/PEG in 5 mm, leading to the formation of an injectable material. In addition, the DA click reaction crosslinking made a hydrogel that has outstanding shape memory and anti-fatigue properties. The storage modulus and breakage strength of the hydrogels were close to 27 kPa and 109.4 kPa, respectively. The compressive strain could reach up to 81.9%. After 10 cycles of a loading and unloading test, the hydrogel still could be loaded by 80 kPa for 1 min and the corresponding deformation could be completely recovered in 1 min after unloading. The ATDC-5 cells were capsulated into the hydrogel bulk in situ and showed high metabolic viability and proliferation. All of these results suggest that the HA/PEG injectable hydrogel formed by integrating the two cross-linking processes has a great potential in cartilage tissue engineering.
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