Journal
POLYMER CHEMISTRY
Volume 5, Issue 12, Pages 3884-3893Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4py00114a
Keywords
-
Categories
Funding
- Grant Agency of the Czech Republic [P208/10/1600]
- Charles University (Prague, CZ)
- TACR [TE01020118]
Ask authors/readers for more resources
A new biocompatible and biodegradable diblock copolymer that contains a specific acid-labile degradable linkage (acyclic ketal group) between the hydrophobic poly(epsilon-caprolactone) (PCL) and the hydrophilic poly(ethylene oxide monomethyl ether) (MPEO) blocks is described herein. A multi-step synthetic method that combines carbodiimide chemistry, a click reaction and ring-opening polymerization (ROP) was employed to successfully produce a series of MPEO-b-PCL diblock copolymers ( herein referred to as MPEO44-b-PCL17 and MPEO44-b-PCL44). 2-{[2-(2-Azidoethoxy) propan-2-yl]} ethan-1-ol was obtained as a linker between the two blocks through a three-step synthetic approach. Furthermore, a newly developed alpha-methoxy- omega-hydroxy-poly(ethylene oxide) that contains an acid-labile ketal linkage was designed as a macroinitiator via a click reaction for the sequential controlled ring-opening polymerization of epsilon-CL. The newly obtained compounds (precursors, macromer, macroinitiator and final diblock copolymers) were assessed by H-1 NMR, C-13 NMR and FT-IR spectroscopy and SEC analysis, which are described in this manuscript. Upon dissolution in a mild organic solvent, the MPEO44-b-PCL17 block copolymer self-assembled in water-PBS into regular, spherical, stable nanoparticles (NPs). Furthermore, the presence of the acid-labile ketal linker enabled the disassembly of these nanoparticles in a buffer that simulated acidic cytosolic or endosomal conditions in tumour cells as evaluated by dynamic light scattering (DLS), nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) images. This disassembly led to hydrolysis profiles that resulted in neutral degradation products.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available