Journal
POLYMER CHEMISTRY
Volume 2, Issue 2, Pages 323-327Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0py00178c
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Funding
- National Science Foundation [CAREER DMR-0846792]
- Alfred P. Sloan Research Fellowship
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Reversible addition-fragmentation chain transfer (RAFT) of N-isopropylacrylamide with a chain transfer agent containing an activated-ester moiety was employed to prepare well-defined temperature-responsive polymers for conjugation to amine-containing proteins. Bioconjugation efficiency of the polymers with lysozyme was investigated as a function of stoichiometry, polymer molecular weight, and reaction pH. The resulting polymer-protein conjugates retained the temperature-responsive nature of the original polymers.
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