Guanidinated multi-arm star polyornithines with a polyethylenimine core for gene delivery

Multi-arm star polyornithines PEI-P(Orn)(n) are prepared by grafting polyornithine arms onto branched polyethylenimine (PEI) with an M-w of 600 via the ring-opening polymerization of N-carboxyanhydride of benzyloxycarbonyl omithine. To enhance gene delivery efficiency and reduce cytotoxicity, the amino side groups on the polyomithine arms are partially guanidinated that transforms the omithine units to arginine units. Thus, the guanidinated products G-PEI-P(Orn)(n) contain multiple poly(ornithine-co-arginine) arms. PEI-P(Orn)(n) and G-PEI-P(Orn)(71) mediate the transfection of pGL3 plasmid to 293T cells almost as efficient as 25 kDa PEI in serum-free medium. Notably, in contrast to the dramatically lowered efficiency of 25 kDa PEI in the presence of serum, the efficiency of G-PEI-P(Orn)(71) can be retained or even enhanced in the medium containing 10% serum. The improved serum-compatibility and high efficiency of the guanidinium-modified star polyornithines make them promising for gene delivery. (C) 2014 Elsevier Ltd. All rights reserved.