Journal
POLYHEDRON
Volume 29, Issue 2, Pages 731-738Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.poly.2009.10.031
Keywords
Amidino-O-alkylurea; Copper(II); DNA binding; DNA cleavage; Nuclease activity; Antibacterial
Categories
Funding
- Center of Excellence for innovation in Chemistry (PERCH-CIC)
- Thailand Research Fund (TRF)
- Thailand Research Fund (TRF), Khon Kaen University and the Development and Promotion of Science and Technology Talents Project
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The two designed coppee(II) complexes, [Cu(L-1m)(2)]Cl-2 (1) (L-1m = amidino-O-methylurea) and [Cu(L-2m)(2)] Cl-2 (2) (L-2m = N-methylphenyl-amidino-o-methylurea), have been investigated for their interaction with calf thymus DNA by utilizing the absorption titration method, viscometric studies and thermal denaturation. The cleavage reaction on pBR322 DNA has been monitored by agarose gel electrophoresis. The results suggest that the two complexes can bind to DNA by non-intercalative modes and exhibit nuclease activities in which supercoiled plasmid DNA is converted to the linear form. Complex 2, with an intrinsic binding constant (K-b) of 1.16 x 10(5) M-1, shows a higher binding efficiency and a better nuclease activity than complex 1, with a K-b value of 5.67 x 10(4) M-1. Their DNA cleavage potential can be significantly enhanced by hydrogen peroxide, indicating an oxidative cleavage process. Further examination of the antibacterial activities against Campylobacter has revealed inhibition zones of 9.0 (for 1) and 14.5 mm (for 2), which are in agreement with their minimum inhibitory concentration (MIC) values of 1.56 and 0.78 mg mL(-1), respectively. The substantially better reactivity of 2 results from the aromatic moieties on the side chain of the L-2m ligand which act as an additional binding site. (C) 2009 Elsevier Ltd. All rights reserved.
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