Journal
POLYHEDRON
Volume 27, Issue 7, Pages 1898-1904Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.poly.2008.02.032
Keywords
artificial nucleases; DNA cleavage; copper complexes; 2,2 '-bipyridine; ammonium groups
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A series of Cu(II) complexes of disubstituted 2,2'-bipyridine bearing ammonium groups [Cu(L1-4)(2)Br](5+) (1-4, L-1 = [5,5'-(Me2NHCH2)(2)-bpy](2+), L-2 = [5,5'-(Me3NCH2)(2)-bpy](2+), L-3 = [4,4'-(Me2NHCH2)(2)-bpy](2+), L-4 = [4,4'-(Me3NCH2)(2)-bpy](2+) and bpy = 2,2'-bipyridyl) were synthesized, of which complexes 1 and 4 were structurally characterized. Both coordination configurations of Cu(II) ions can be described as distorted trigonal bipyramid. The interaction between all complexes and CT-DNA was evaluated by thermal-denaturation experiments and CD spectroscopy. Results show that the complexes interact with CT-DNA via outside electrostatic interactions and their binding ability follows the order: 1 > 2 > 3 > 4. In the absence of any reducing agents, the cleavage of plasmid pBR322 DNA by these complexes was investigated and the hydrolysis kinetics of DNA was studied in Tris buffer (pH 7.5) at 37 degrees C. Obtained pseudo-Michaelis-Menten kinetic parameters: 15.0, 13.6, 2.01 and 1.69 h(-1) for 1, 2, 3 and 4, respectively, indicate that complexes 1 and 2 exhibit very. high DNA cleavage activities: According to their crystal data, the high nuclease activity may be attributed to the strong interaction. of the metal moiety and two ammonium groups with phosphate groups of DNA. (C) 2008 Elsevier Ltd. All rights reserved.
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