Journal
PLOS ONE
Volume 12, Issue 8, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0183479
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Funding
- Korea Healthcare technology RD Project [HI15C1972010015]
- Ministry for Health, Welfare & Family Affairs, Republic of Korea
- Basic Science Research Program through National Research Foundation of Korea Grants - Korean Government, Republic of Korea [2009-0093821, 2014R1A2A2A01003994, 2015R1D1A1A09057432]
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education, Science, and Technology [2013R1A1A2009661]
- National Research Foundation of Korea [2013R1A1A2009661, 2014R1A2A2A01003994, 2015R1D1A1A09057432] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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MicroRNAs post-transcriptionally regulate gene expression in animals and plants. The aim of this study was to identify new target genes for microRNA polymorphisms (miR-146aC>G and miR-196a2T>C) in primary ovarian insufficiency (POI). We cloned and transfected miR146aC>G and miR-196a2T>C into human granulosa cells and used microarrays and qPCR-arrays to examine the changes in the messenger RNA expression profile. We show miR146aC>G and miR-196a2T>C change the mRNA expression patterns in granulosa cell. In each case, mRNAs were up or down-regulated after treatments with miR-146a C or G and miR-196a2 T or C. We found that miR-146a led to a significantly altered regulation of the mRNA levels of FOXO3, FOXL2 and CCND2 compared to controls. We also found that the polymorphisms of miR-146a led to a significantly altered regulation of CCND2 and FOXO3. Our results suggest that miR-146aC>G and miR-196a2T>C can regulate the levels of many of their target transcripts. In addition, specific target genes of miR-146aC>G polymorphisms may be involved in granulosa cell regulation.
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