Journal
CHEMICAL SCIENCE
Volume 6, Issue 1, Pages 301-307Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4sc02600d
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Funding
- National Basic Research Program of China [2012CB933301]
- National Natural Science Foundation of China [21171098]
- Program for New Century Excellent Talents in University [NCET-12-0740]
- Ministry of Education of China [IRT1148, 20133223110006]
- Natural Science Foundation of Jiangsu Province of China [BK20130038]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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We report a ratiometric phosphorescence sensory system for hypochlorite (ClO-) based on core-shell structured silica nanoparticles. Two phosphorescent iridium(III) complexes were immobilised in the inner solid core and outer mesoporous layer of the nanoparticles, respectively. The former is insensitive to ClO- and thus serves as an internal standard to increase the accuracy and precision, while the latter exhibits a specific and significant luminogenic response to ClO-, providing high selectivity and sensitivity. Upon exposure to ClO-, the nanoparticles display a sharp luminescence colour change from blue to red. Additionally, intracellular detection of exogenous and endogenous ClO- has been demonstrated via ratiometric imaging and photoluminescence lifetime imaging microscopy. Compared to intensity-based sensing, ratiometric and lifetime-based measurements are independent of the probe concentration and are thus less affected by external influences, especially in intracellular applications.
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