Journal
PLOS ONE
Volume 11, Issue 4, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0153137
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Funding
- National Institute of Allergy and Infectious Diseases [R01AI0988530]
- U.S. Department of Energy's National Nuclear Security Administration [DE-AC04-94AL85000]
- NIH-NIAID [R01AI0988530]
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We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (similar to 5 mu L). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.
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