Journal
PLOS ONE
Volume 11, Issue 2, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0149478
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Funding
- Polish National Science Center [UMO-2014/13/B/ST5/04144, UMO-2013/08/A/ST5/00295, UMO-2013/10/E/NZ1/00741, UMO-2012/05/D/ST5/03599]
- Polish Ministry of Science and Higher Education, under the KNOW program
- Institute of Bioorganic Chemistry for young researchers and PhD students
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This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in beta-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the same as that of naturally occurring D-RNA counterparts. In addition, formation of D-RNA/L-RNA heterochiral duplexes is also observed; however, their thermodynamic stability is significantly reduced in reference to homochiral D-RNA duplexes. The presence of three locked nucleic acid (LNA) residues within the D-RNA strand diminishes the negative effect of the enantiomeric, complementary L-RNA strand in the formation of heterochiral RNA duplexes. Similar behavior is also observed for heterochiral LNA-2'-O-methyl- D-RNA/L-RNA duplexes. The formation of heterochiral duplexes was confirmed by H-1 NMR spectroscopy. The CD curves of homochiral L-RNA structural motifs are always reversed, whereas CD curves of heterochiral duplexes present individual features dependent on the composition of chiral strands.
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