4.6 Article

Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size

Journal

PLOS ONE
Volume 11, Issue 1, Pages -

Publisher

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0147235

Keywords

-

Funding

  1. Japan Society for Promotion of Science [25293413, 2643017]
  2. Grants-in-Aid for Scientific Research [25293413] Funding Source: KAKEN

Ask authors/readers for more resources

Objectives This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. Materials and Methods Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 mu m. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. Results Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 mu m. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. Conclusions PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available