Journal
PLOS ONE
Volume 11, Issue 1, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0146120
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Funding
- National Institutes of Health (NIH) [3 R01 GM45735, 1 R01 GM096445, SC1 GM088114, 5 T32 DK7647, 5 T32 GM007367]
- Ellison Medical Foundation [AG-SS-2465-10]
- Research Centers in Minority Institutions Program from National Institute on Minority Health and Health Disparities [MD007599]
- NIH/NCI [5 P30 CA13696]
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Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging.
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