Journal
PLOS ONE
Volume 10, Issue 9, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0138876
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Funding
- MEXT KAKENHI [23012025, 25113009, 25119711, 15H01233]
- JSPS KAKENHI [24580140, 24570048, 26650095]
- JSPS [21-764, 24-7049]
- Asahi Glass Foundation
- SUNTORY Foundation for Life Sciences
- Grants-in-Aid for Scientific Research [26650095, 24570048, 15H01233, 23012025, 25113009, 24580140, 15H04391, 26650111] Funding Source: KAKEN
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We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3xCitrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3xFLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.
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