Journal
PLOS ONE
Volume 10, Issue 4, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0118803
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Funding
- National Institutes of Health Grant [R01GM101420-01A1]
- Kathy and Curt Marble Cancer Research Fund
- Ragon Institute of Harvard/MIT/MGH
- National Cancer Institute Cancer Center Support (Core) Grant [P30-CA14051]
- Mazumdar-Shaw International Oncology Fellowship
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Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.
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