Journal
PLOS ONE
Volume 10, Issue 4, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0121683
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Categories
Funding
- Engineering and Physical Sciences Research Council
- Higher Education Commission of Pakistan
- Biotechnology and Biological Sciences Research Council (David Phillips Fellowship) [BB/D020638/1]
- Medical Research Council [MR/K015931/1]
- Biotechnology and Biological Sciences Research Council [BB/D020638/1] Funding Source: researchfish
- Medical Research Council [MR/K015931/1] Funding Source: researchfish
- BBSRC [BB/D020638/1] Funding Source: UKRI
- MRC [MR/K015931/1] Funding Source: UKRI
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The methods currently available to deliver functional labels and drugs to the cell cytosol are inefficient and this constitutes a major obstacle to cell biology (delivery of sensors and imaging probes) and therapy (drug access to the cell internal machinery). As cell membranes are impermeable to most molecular cargos, viral peptides have been used to bolster their internalisation through endocytosis and help their release to the cytosol by bursting the endosomal vesicles. However, conflicting results have been reported on the extent of the cytosolic delivery achieved. To evaluate their potential, we used gold nanoparticles as model cargos and systematically assessed how the functionalisation of their surface by either or both of the viral peptides TAT and HA2 influenced their intracellular delivery. We evaluated the number of gold nanoparticles present in cells after internalisation using photothermal microscopy and their subcellular localisation by electron microscopy. While their uptake increased when the TAT and/or HA2 viral peptides were present on their surface, we did not observe a significant cytosolic delivery of the gold nanoparticles.
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