Journal
PLOS ONE
Volume 9, Issue 6, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0099257
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Funding
- National Agency for the Promotion of Science and Technology of Argentina (ANPCYT) [BID OC-AR 1728, PICT 2004-13970, PICT 2007-00628]
- National Research Council of Argentina (CONICET) [PIP 11220080 102551]
- Pontifical Catholic University of Argentina (UCA)
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Patients with cystic fibrosis (CF) have elevated concentration of cytokines in sputum and a general inflammatory condition. In addition, CF cells in culture produce diverse cytokines in excess, including IL-1 beta. We have previously shown that IL-1 beta, at low doses (similar to 30 pM), can stimulate the expression of CFTR in T84 colon carcinoma cells, through NF-kappa B signaling. However, at higher doses (similar to 2.5 ng/ml, similar to 150 pM), IL-1 beta inhibit CFTR mRNA expression. On the other hand, by using differential display, we found two genes with reduced expression in CF cells, corresponding to the mitochondrial proteins CISD1 and MTND4. The last is a key subunit for the activity of mitochondrial Complex I (mCx-I); accordingly, we later found a reduced mCx-I activity in CF cells. Here we found that IB3-1 cells (CF cells), cultured in serum-free media, secrete 323 +/- 5 pg/ml of IL-1 beta in 24 h vs 127 +/- 3 pg/ml for S9 cells (CFTR-corrected IB3-1 cells). Externally added IL-1 beta (5 ng/ml) reduces the mCx-I activity and increases the mitochondrial (MitoSOX probe) and cellular (DCFH-DA probe) ROS levels of S9 (CFTR-corrected IB3-1 CF cells) or Caco-2/pRSctrl cells (shRNA control cells) to values comparable to those of IB3-1 or Caco-2/pRS26 cells (shRNA specific for CFTR). Treatments of IB3-1 or Caco-2/pRS26 cells with either IL-1b blocking antibody, IL-1 receptor antagonist, IKK inhibitor III (NF-kappa B pathway) or SB203580 (p38 MAPK pathway), restored the mCx-I activity. In addition, in IB3-1 or Caco-2/pRS26 cells, IL-1b blocking antibody, IKK inhibitor III or SB203580 reduced the mitochondrial ROS levels by,50% and the cellular ROS levels near to basal values. The AP-1 inhibitors U0126 (MEK1/2) or SP600125 (JNK1/2/3 inhibitor) had no effects. The results suggest that in these cells IL-1 beta, through an autocrine effect, acts as a bridge connecting the CFTR with the mCx-I activity and the ROS levels.
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