4.6 Article

Prolactin Induces Apoptosis of Lactotropes in Female Rodents

Journal

PLOS ONE
Volume 9, Issue 5, Pages -

Publisher

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0097383

Keywords

-

Funding

  1. Agencia Nacional de Promocion de Ciencia y Tecnica (ANPCYT) [PICT 092, PICT 0088]
  2. Consejo Nacional de Investigaciones Cientificas y Tecnologicas (CONICET)
  3. University of Buenos Aires, Argentine [UBACYT M057]
  4. INSERM-CONICET, Argentina
  5. INSERM-CONICET, France
  6. Association for International Cancer Research (AICR) [05-0603]
  7. Institut National de la Sante et de la Recherche Medicale
  8. University Paris Descartes, France
  9. ANPCyT (Argentina)
  10. Consejo Nacional de Investigaciones Cientificas y Tecnologicas (CONICET) (Argentina)
  11. Bourse Doctorale Universitaire de Mobilite from the Universite Paris Descartes (France)

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Anterior pituitary cell turnover occurring during female sexual cycle is a poorly understood process that involves complex regulation of cell proliferation and apoptosis by multiple hormones. In rats, the prolactin (PRL) surge that occurs at proestrus coincides with the highest apoptotic rate. Since anterior pituitary cells express the prolactin receptor (PRLR), we aimed to address the actual role of PRL in the regulation of pituitary cell turnover in cycling females. We showed that acute hyperprolactinemia induced in ovariectomized rats using PRL injection or dopamine antagonist treatment rapidly increased apoptosis and decreased proliferation specifically of PRL producing cells (lactotropes), suggesting a direct regulation of these cell responses by PRL. To demonstrate that apoptosis naturally occurring at proestrus was regulated by transient elevation of endogenous PRL levels, we used PRLR-deficient female mice (PRLRKO) in which PRL signaling is totally abolished. According to our hypothesis, no increase in lactotrope apoptotic rate was observed at proestrus, which likely contributes to pituitary tumorigenesis observed in these animals. To decipher the molecular mechanisms underlying PRL effects, we explored the isoform-specific pattern of PRLR expression in cycling wild type females. This analysis revealed dramatic changes of long versus short PRLR ratio during the estrous cycle, which is particularly relevant since these isoforms exhibit distinct signaling properties. This pattern was markedly altered in a model of chronic PRLR signaling blockade involving transgenic mice expressing a pure PRLR antagonist (TG(Delta 1-9-G129R-hPRL)), providing evidence that PRL regulates the expression of its own receptor in an isoform-specific manner. Taken together, these results demonstrate that i) the PRL surge occurring during proestrus is a major proapoptotic signal for lactotropes, and ii) partial or total deficiencies in PRLR signaling in the anterior pituitary may result in pituitary hyperplasia and eventual prolactinoma development, as observed in TG(Delta 1-9-G129R-hPRL) and PRLRKO mice, respectively.

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