Journal
PLOS ONE
Volume 9, Issue 6, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0100328
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Funding
- German Federal Ministry of Education and Research [BMBF 01 EO 0803]
- German Research Foundation (DFG) [SFB620]
- 7th Framework Program (FP7) of the European Union [HEALTH-F2-2008-201549]
- FP7-E-Rare GENTHALTHER''
- Virginia Commonwealth University (VCU) Presidential Research Incentive Program (PRIP)
- VCU Massey Cancer Center/American Cancer Society Institutional Research Awards
- NIH [HD076257]
- University Freiburg (UF) Medical Faculty Research Committee
- UF Scientific Society Freiburg Research Grants
- PERSIST [FP7-HEALTH-2007-B/222878]
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Currently very little is known about the differential expression and function of the transcription factor SOX5 during B cell maturation. We identified two new splice variants of SOX5 in human B cells, encoding the known L-SOX5B isoform and a new shorter isoform L-SOX5F. The SOX5 transcripts are highly expressed during late stages of B-cell differentiation, including atypical memory B cells, activated CD21(low) B cells and germinal center B cells of tonsils. In tonsillar sections SOX5 expression was predominantly polarized to centrocytes within the light zone. After in vitro stimulation, SOX5 expression was down-regulated during proliferation while high expression levels were permissible for plasmablast differentiation. Overexpression of L-SOX5F in human primary B lymphocytes resulted in reduced proliferation, less survival of CD138(neg) B cells, but comparable numbers of CD138(+)CD38(hi) plasmablasts compared to control cells. Thus, our findings describe for the first time a functional role of SOX5 during late B cell development reducing the proliferative capacity and thus potentially affecting the differentiation of B cells during the germinal center response.
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