Journal
PLOS ONE
Volume 9, Issue 6, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0101277
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Categories
Funding
- 973-plan [2012CB720800]
- National Natural Science Foundation of China [31101278, 31130042]
- national science and technology support program [2012BAD37B01]
- Research Fund for the Doctoral Program of Higher Education of China [20110172120033]
- YangFan Innovative & Entepreneurial Research Team Project [201312H05]
- Fundamental Research Funds for the Central Universities [2013ZZ0069]
- Science and technology plan of Guangdong Province [2012B091100075]
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This study was to investigate the synergistic effect of NB/Cur on growth and apoptosis in A375 human melanoma cell line by MTT assay, flow cytometry and Western blotting. Our results demonstrated that NB effectively synergized with Cur to enhance its antiproliferative activity on A375 human melanoma cells by induction of apoptosis, as evidenced by an increase in sub-G1 cell population, DNA fragmentation, PARP cleavage and caspase activation. Further mechanistic studies by Western blotting showed that after treatment of the cells with NB/Cur, up-regulation of the expression level of phosphorylated JNK and down-regulation of the expression level of phosphorylated ERK and Akt contributed to A375 cells apoptosis. Moreover, NB also potentiated Cur to trigger intracellular ROS overproduction and the DNA damage with up-regulation of the expression level of phosphorylated ATM, phosphorylated Brca1 and phosphorylated p53. The results indicate the combinational application potential of NB and Cur in treatments of cancers.
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