Journal
PLOS ONE
Volume 9, Issue 5, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0096162
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Funding
- Chinese National Key Technologies R&D Program of 12th Five-year Plan [2012BAD31B06]
- Province Key Science and Technology Innovation Team Project [2010R50032, 2012T2T123]
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Proanthocyanidins in Chinese bayberry leaves (PCBLs) were qualitatively analyzed. NMR data suggest that PCBLs are mostly composed of (epi) gallocatechin gallate units. Matrix-assisted laser desorption time-of-flight MS data indicate 95 possible prodelphinidin structures, ranging from dimers to tridecamers. Preparative normal-phase HPLC and further analysis by reverse-phase HPLC together with electrospray ionization MS enabled detection of 20 compounds, including seven newly identified compounds in Chinese bayberry leaves. The antioxidant capacity of PCBLs was evaluated by (1,1-diphenyl-2-picryl-hydrazyl), ferric-reducing antioxidant power, and oxygen radical absorption capacity assays. The EC50 of DPPH radical scavenging activities (as 50% decrease in the initial DPPH concentration) were 7.60 mu g. The FRAP and ORAC values were 8859.336 +/- 978.39 and 12991.61 +/- 1553.34 mu mol Trolox equivalents per gram, respectively. The results indicate the high antioxidant potency of PCBLs.
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