Journal
PLOS ONE
Volume 9, Issue 1, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0086372
Keywords
-
Categories
Funding
- ERC starting grant
- Cambridge Hospitals National Institute for Health Research Biomedical Research Center
- NIH-Oxford Cambridge Scholars Program Fellowship
- Evelyn trust
- EU grant InnovaLiv
- EPSRC [DT/E005039/2] Funding Source: UKRI
- MRC [G0701448] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [1085978] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [DT/E005039/2] Funding Source: researchfish
- Medical Research Council [G0701448] Funding Source: researchfish
Ask authors/readers for more resources
Induced pluripotent stem cell derived hepatocytes (IPSC-Heps) have the potential to reduce the demand for a dwindling number of primary cells used in applications ranging from therapeutic cell infusions to in vitro toxicology studies. However, current differentiation protocols and culture methods produce cells with reduced functionality and fetal-like properties compared to adult hepatocytes. We report a culture method for the maturation of IPSC-Heps using 3-Dimensional (3D) collagen matrices compatible with high throughput screening. This culture method significantly increases functional maturation of IPSC-Heps towards an adult phenotype when compared to conventional 2D systems. Additionally, this approach spontaneously results in the presence of polarized structures necessary for drug metabolism and improves functional longevity to over 75 days. Overall, this research reveals a method to shift the phenotype of existing IPSC-Heps towards primary adult hepatocytes allowing such cells to be a more relevant replacement for the current primary standard.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available