Acclimatization of the Crustose Coralline Alga Porolithon onkodes to Variable pCO2

Ocean acidification (OA) has important implications for the persistence of coral reef ecosystems, due to potentially negative effects on biomineralization. Many coral reefs are dynamic with respect to carbonate chemistry, and experience fluctuations in pCO(2) that exceed OA projections for the near future. To understand the influence of dynamic pCO(2) on an important reef calcifier, we tested the response of the crustose coralline alga Porolithon onkodes to oscillating pCO(2). Individuals were exposed to ambient (400 mu atm), high (660 mu atm), or variable pCO(2) (oscillating between 400/660 mu atm) treatments for 14 days. To explore the potential for coralline acclimatization, we collected individuals from low and high pCO(2) variability sites (upstream and downstream respectively) on a back reef characterized by unidirectional water flow in Moorea, French Polynesia. We quantified the effects of treatment on algal calcification by measuring the change in buoyant weight, and on algal metabolism by conducting sealed incubations to measure rates of photosynthesis and respiration. Net photosynthesis was higher in the ambient treatment than the variable treatment, regardless of habitat origin, and there was no effect on respiration or gross photosynthesis. Exposure to high pCO(2) decreased P. onkodes calcification by >70%, regardless of the original habitat. In the variable treatment, corallines from the high variability habitat calcified 42% more than corallines from the low variability habitat. The significance of the original habitat for the coralline calcification response to variable, high pCO(2) indicates that individuals existing in dynamic pCO(2) habitats may be acclimatized to OA within the scope of in situ variability. These results highlight the importance of accounting for natural pCO(2) variability in OA manipulations, and provide insight into the potential for plasticity in habitat and species-specific responses to changing ocean chemistry.