Journal
PLOS ONE
Volume 8, Issue 12, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0083044
Keywords
-
Categories
Funding
- Chinese National Natural Science Foundation [30830021, 31070701, 31000960, 31025025, U1132601, 31200590]
- Ministry of Science and Technology [2010CB529800, 2011ZX09102-002-10]
- Yunnan Province [2011CI139, 2012BC009, 2013FB072]
- Jiangsu Province [BK2012365, BE2012748]
Ask authors/readers for more resources
Antimicrobial peptides have been widely identified from amphibian skins except salamanders. A novel antimicrobial peptide (CFBD) was isolated and characterized from skin secretions of the salamander, Cynops fudingensis. The cDNA encoding CFBD precursor was cloned from the skin cDNA library of C. fudingensis. The precursor was composed of three domains: signal peptide of 17 residues, mature peptide of 41 residues and intervening propeptide of 3 residues. There are six cysteines in the sequence of mature CFBD peptide, which possibly form three disulfide-bridges. CFBD showed antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Candida albicans and Escherichia coli. This peptide could be classified into family of beta-defensin based on its seqeuence similarity with beta-defensins from other vertebrates. Evolution analysis indicated that CFBD was close to fish beta-defensin. As far as we know, CFBD is the first beta-defensin antimicrobial peptide from salamanders.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available