Journal
PLOS ONE
Volume 8, Issue 7, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0068990
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Funding
- Ministry of Science and Technology [2011CB965100, 2011DFA30480, 2010CB944900, 2010CB945000, 2012CB966603, 2011CBA01100, 2013CB967401, 2013CB531606]
- National Natural Science Foundation of China [31210103905, 91219305, 31201107, 31101061, 81170499, 31071306, 31000378, 31171432, 81273282]
- Science and Technology Commission of Shanghai Municipality [12ZR1450900, 11ZR1438500, 11XD1405300, 11JC1410902]
- Ministry of Education [IRT1168, 20110072110039]
- Fundamental Research Funds for the Central Universities [2000219066, 2000219067, 2000219077]
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The mechanisms by which microRNAs (miRNAs) affect cell fate decisions remain poorly understood. Herein, we report that miR-200a can suppress the differentiation of mouse embryonic stem (ES) cells into endoderm and mesoderm. Interestingly, miR-200a directly targets growth factor receptor-bound protein 2 (Grb2), which is a key adaptor in the Erk signaling pathway. Furthermore, high levels of miR-200a dramatically decrease Grb2 levels and suppress the appearance of mesoderm and endoderm lineages in embryoid body formation, as well as suppressing the activation of Erk. Finally, Grb2 supplementation significantly rescues the miR-200a-induced layer-formation bias and the Erk suppression. Collectively, our results demonstrate that miR-200a plays critical roles in ES cell lineage commitment by directly regulating Grb2 expression and Erk signaling.
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