Journal
PLOS ONE
Volume 8, Issue 7, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0070374
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Funding
- Fondazione Banco di Sardegna, Sassari, Italy
- Ministero dell'Universita e della Ricerca, Italy
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Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma. After derivatization of L-ergothioneine with 5-iodoacetamidofluorescein, the separation was carried out by HPLC on an ODS-2 C-18 sperisorb column by using a linear gradient elution and by HPCE on an uncoated fused silica capillary, 50 mu m id, and 60 cm length. The methods were validated and found to be linear in the range of 0.3 to 10 mu mol/l. The limit of quantification was 0.27 mu mol/l for HPCE and 0.15 mu mol/l for HPLC. The variations for intra-and inter-assay precision were around 6 RSD%, and the mean recovery accuracy close to 100% (96.11%).
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