Retinal Thickness Normative Data in Wild-Type Mice Using Customized Miniature SD-OCT

Objective: To report normative data for retinal thickness in wild-type C57BL/6 mouse utilizing a miniature SD-OCT system. Methods: Thirty adult mice (range: 3-5 months) were anesthetized and secured into the Bioptigen Spectral Domain Ophthalmic Imaging System. Right eye SD-OCT images were standardized by centralizing the optic nerve head (ONH) prior to image acquisition. Global and quadrant total retinal thickness (TRT) values were measured from retinal nerve fiber layer to retinal pigment epithelial layer. Posterior segment analyses also included the outer retinal layer (ORL) and inner retinal layer (IRL). Further sublayer analyses of four layers from the ORL and three layers comprising the IRL were also performed. Results: The overall mean +/- SD global TRT in a C57BL/6 mouse model was 204.41 +/- 5.19 mu m. Quadrant mean TRT values were 204.85 +/- 5.81 mu m inferiorly, 204.97 +/- 6.71 mu m nasally, 205.08 +/- 5.44 mu m temporally, and 202.74 +/- 4.85 mu m superiorly. Mean +/- SD thickness for ORL, and IRL were 126.37 +/- 10.01 mu m, and 107.03 +/- 10.98 mu m respectively. The mean +/- SD estimates for the four layers of the ORL were 18.23 +/- 2.73 mu m, 26.04 +/- 4.21 mu m, 63.8 +/- 6.23 mu m, and 19.22 +/- 4.34 mu m. Mean +/- SD values for the three IRL sublayers were 27.82 +/- 4.04 mu m, 59.62 +/- 6.66 mu m and 19.12 +/- 3.71 mu m. Conclusion: This study established normative values for the total retinal thickness and sublayer thickness for the wild-type C57BL/6 mice. Moreover, it provides a standard of retinal morphology, in a commonly used animal model, for evaluating therapeutic interventions and retinal disease pathophysiology.