Journal
PLOS ONE
Volume 8, Issue 1, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0053015
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Funding
- Pediatric Brain Tumor Foundation of the United States
- Brain Tumor Society
- Goldhirsh Foundation
- James D. McDonnell Foundation
- National Institutes of Health [CA112958, CA116659, CA154130]
- Pathway to Independence Award [CA157948]
- Case Western Reserve Medical Scientist Training Program [T32 GM007250]
- National Research Service Award, National Cancer Institute [F30 CA165857]
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Colon cancer is a deadly disease affecting millions of people worldwide. Current treatment challenges include management of disease burden as well as improvements in detection and targeting of tumor cells. To identify disease state-specific surface antigen signatures, we combined fluorescent cell barcoding with high-throughput flow cytometric profiling of primary and metastatic colon cancer lines (SW480, SW620, and HCT116). Our multiplexed technique offers improvements over conventional methods by permitting the simultaneous and rapid screening of cancer cells with reduced effort and cost. The method uses a protein-level analysis with commercially available antibodies on live cells with intact epitopes to detect potential tumor-specific targets that can be further investigated for their clinical utility. Multiplexed antibody arrays can easily be applied to other tumor types or pathologies for discovery-based approaches to target identification.
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