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PLOS ONE
Volume 7, Issue 11, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0049579
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We have previously shown that the HER2-specific affibody molecule (Z(HER2:342))(2) inhibits proliferation of SKBR-3 cells. Here, we continue to investigate its biological effects in vitro by studying receptor dimerization and clonogenic survival following irradiation. We found that (Z(HER2:342))(2) sensitizes the HER2-overexpressing cell line SKBR-3 to ionizing radiation. The survival after exposure to (Z(HER2:342))(2) and 8 Gy (S-8Gy 0.006) was decreased by a factor four compared to the untreated (S-8Gy 0.023). The low HER2-expressing cell line MCF-7 was more radiosensitive than SKBR-3 but did not respond to (Z(HER2:342))(2).Treatment by (Z(HER2:342))(2) strongly increased the levels of dimerized and phosphorylated HER2 even after 5 minutes of stimulation. The monomeric Z(HER2:342) does not seem to be able to induce receptor phosphorylation and dimerization or sensitize cells to irradiation.
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