Journal
PLOS ONE
Volume 7, Issue 5, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0036397
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Funding
- MRC (Medical Research Council) doctoral training grant
- BMBF (Bundesministerium fur Bildung und Forschung) in the NGFN2 (Das Nationale Genomforschungsnetz)
- NGFNplus Parkinson programs
- Medical Research Council [MR/J004324/1, G0700932] Funding Source: researchfish
- Parkinson's UK [G-0808, G-0803, H-1003, G-1103] Funding Source: researchfish
- MRC [G0700932, MR/J004324/1] Funding Source: UKRI
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Mutations in, or elevated dosage of, SNCA, the gene for alpha-synuclein (alpha-syn), cause familial Parkinson's disease (PD). Mouse lines overexpressing the mutant human A53T alpha-syn may represent a model of early PD. They display progressive motor deficits, abnormal cellular accumulation of alpha-syn, and deficits in dopamine-dependent corticostriatal plasticity, which, in the absence of overt nigrostriatal degeneration, suggest there are age-related deficits in striatal dopamine (DA) signalling. In addition A53T alpha-syn overexpression in cultured rodent neurons has been reported to inhibit transmitter release. Therefore here we have characterized for the first time DA release in the striatum of mice overexpressing human A53T alpha-syn, and explored whether A53T alpha-syn overexpression causes deficits in the release of DA. We used fast-scan cyclic voltammetry to detect DA release at carbon-fibre microelectrodes in acute striatal slices from two different lines of A53T alpha-syn-overexpressing mice, at up to 24 months. In A53T alpha-syn overexpressors, mean DA release evoked by a single stimulus pulse was not different from wild-types, in either dorsal striatum or nucleus accumbens. However the frequency responsiveness of DA release was slightly modified in A53T alpha-syn overexpressors, and in particular showed slight deficiency when the confounding effects of striatal ACh acting at presynaptic nicotinic receptors (nAChRs) were antagonized. The re-release of DA was unmodified after single-pulse stimuli, but after prolonged stimulation trains, A53T alpha-syn overexpressors showed enhanced recovery of DA release at old age, in keeping with elevated striatal DA content. In summary, A53T alpha-syn overexpression in mice causes subtle changes in the regulation of DA release in the striatum. While modest, these modifications may indicate or contribute to striatal dysfunction.
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