Journal
PLOS ONE
Volume 7, Issue 2, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0030503
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Funding
- National Natural Science Foundation of China [30900620, 81000070]
- Science Foundation of the Third Military Medical University [2009XQN33]
- Natural Foundation of Chongqing [CSTC,2009BB5020]
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The proliferation, migration, and angiogenesis of endothelial progenitor cells (EPCs) play critical roles in postnatal neovascularization and re-endothelialization following vascular injury. Here we evaluated whether the over-expression of platelet-derived growth factor receptor-beta (PDGFR-beta) can enhance the PDGF-BB-stimulated biological functions of EPCs through the PDGFR-beta/phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. We first confirmed the expression of endogenous PDGFR-beta and its plasma membrane localization in spleen-derived EPCs. We then demonstrated that the PDGFR-beta over-expression in EPCs enhanced the PDGF-BB-induced proliferation, migration, and angiogenesis of EPCs. Using AG1295 (a PDGFR kinase inhibitor), LY294002 (a PI3K inhibitor), and sc-221226 (an Akt inhibitor), we further showed that the PI3K/Akt signaling pathway participates in the PDGF-BB-induced proliferation, migration, and angiogenesis of EPCs. In addition, the PI3K/Akt signaling pathway is required for PDGFR-beta over-expression to enhance these PDGF-BB-induced phenotypes.
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