Journal
PLOS ONE
Volume 6, Issue 11, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0027805
Keywords
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Categories
Funding
- Medical Research Centre
- Wellcome Trust [WT081703MA]
- European Community [FP7/2007-2013]
- EC [223498]
- Medical Research Council Centre for Molecular Medical Virology [G07008]
- UCL/UCLH National Institute for Health Research Comprehensive Biomedical Research Centre
- Wellcome Trust Sanger Institute
- Medical Research Council [G0700814, G0900950] Funding Source: researchfish
- MRC [G0700814, G0900950] Funding Source: UKRI
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Whole genome sequencing of viruses directly from clinical samples is integral for understanding the genetics of host-virus interactions. Here, we report the use of sample sparing target enrichment (by hybridisation) for viral nucleic acid separation and deep-sequencing of herpesvirus genomes directly from a range of clinical samples including saliva, blood, virus vesicles, cerebrospinal fluid, and tumour cell lines. We demonstrate the effectiveness of the method by deep-sequencing 13 highly cell-associated human herpesvirus genomes and generating full length genome alignments at high read depth. Moreover, we show the specificity of the method enables the study of viral population structures and their diversity within a range of clinical samples types.
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