Journal
PLOS ONE
Volume 6, Issue 8, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0023160
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Funding
- National Institutes of Health [HL031607-28, PO1 HL 068758, R37 HL104017, HL007501-26, K08HL071563]
- Wellcome Trust [085483/Z/08/Z]
- Wellcome Trust [085483/Z/08/Z] Funding Source: Wellcome Trust
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Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.
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