Journal
PLOS ONE
Volume 5, Issue 12, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0014481
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Funding
- Paul Blumel Foundation
- Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG 2009, IG 5697]
- Telethon [GGP07177]
- Progetto PRIN
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Here, we show that the eukaryotic translation elongation factor 1 gamma (eEF1 gamma) physically interacts with the RNA polymerase II (pol II) core subunit 3 (RPB3), both in isolation and in the context of the holo-enzyme. Importantly, eEF1 gamma has been recently shown to bind Vimentin mRNA. By chromatin immunoprecipitation experiments, we demonstrate, for the first time, that eEF1 gamma is also physically present on the genomic locus corresponding to the promoter region of human Vimentin gene. The eEF1 gamma depletion causes the Vimentin protein to be incorrectly compartmentalised and to severely compromise cellular shape and mitochondria localisation. We demonstrate that eEF1 gamma partially colocalises with the mitochondrial marker Tom20 and that eEF1 gamma depletion increases mitochondrial superoxide generation as well as the total levels of carbonylated proteins. Finally, we hypothesise that eEF1 gamma, in addition to its role in translation elongation complex, is involved in regulating Vimentin gene by contacting both pol II and the Vimentin promoter region and then shuttling/nursing the Vimentin mRNA from its gene locus to its appropriate cellular compartment for translation.
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