Targeted Amino-Terminal Acetylation of Recombinant Proteins in E. coli

One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co-expressing the fission yeast NatB complex with the target protein in E. coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.