Quadrupled Hamstring Graft Strength as a Function of Clinical Sizing

Purpose: This study sought to compare the strength of quadrupled hamstring tendon (QHT) grafts of 6 to 9.5 mm in clinical diameter with that of 10-mm bone-patellar tendon-bone (BPTB) grafts. Methods: Twenty cadaveric semitendinosus and gracilis tendons were combined into QHT grafts. These were sized using a standard graft-sizing device and an area micrometer, yielding grafts ranging from 6 to 9.5 mm in diameter. The grafts were tested to failure. Five 10-mm BPTB grafts were also sized and tested. Results: Clinical sizing did predict the strength of the graft but not profoundly. As a material alone, without consideration of fixation in bone tunnels, QHT grafts were stronger than BPTB grafts. Graft strength decreased with size, but a linear relation between strength and diameter (r(2) = 0.715, P < .001) was found to be as good as the expected quadratic fit (r(2) = 0.709). Compared with BPTB grafts, even the smallest QHT grafts (diameter < 6.5 mm) were still significantly stronger than 10- mm BPTB grafts (P = .004). The elastic moduli of the QHT and BPTB grafts were 761 +/- 187 MPa and 615 +/- 403 MPa, respectively; elongations at failure were 12.0% +/- 2.0% and 7.5% +/- 1.6%, respectively; and failure stresses were 105 +/- 18 MPa and 50 +/- 14 MPa, respectively. Conclusions: This work shows that a clinical size of QHT grafts of 6 mm in diameter is not a concern regarding the strength itself. For a possible lower-end prediction of acceptable size, assuming that a gracilis-semitendinosus graft would have only the stress of the weakest measured QHT graft of 88 MPa, a graft of 5.5 mm in diameter would suffice, having more strength in newtons than the average patellar tendon.