Journal
PLOS ONE
Volume 4, Issue 8, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0006608
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Funding
- National Heart, Lung and Blood Institute [19278]
- National Center for Research Resources (NIH) [5UL1RR024143]
- New York City Community Trust Career Development Grant Award
- Monique Weill-Caulier Trust New Faculty Research Award
- Stony Brook University
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Thrombospondin 1 (TSP-1), which is contained in platelet alpha-granules and released with activation, has been shown to activate latent TGF-beta 1 in vitro, but its in vivo role is unclear as TSP-1-null (Thbs1(-/-)) mice have a much less severe phenotype than TGF-beta 1-null (Tgfb1(-/-)) mice. We recently demonstrated that stirring and/or shear could activate latent TGF-beta 1 released from platelets and have now studied these methods of TGF-beta 1 activation in samples from Thbs1(-/-) mice, which have higher platelet counts and higher levels of total TGF-beta 1 in their serum than wild type mice. After either two hours of stirring or shear, Thbs1(-/-) samples demonstrated less TGF-beta 1 activation (31% and 54% lower levels of active TGF-beta 1 in serum and platelet releasates, respectively). TGF-beta 1 activation in Thbs1(-/-) mice samples was normalized by adding recombinant human TSP-1 (rhTSP-1). Exposure of platelet releasates to shear for one hour led to near depletion of TSP-1, but this could be prevented by preincubating samples with thiol-reactive agents. Moreover, replenishing rhTSP-1 to human platelet releasates after one hour of stirring enhanced TGF-beta 1 activation. In vivo TGF-beta 1 activation in carotid artery thrombi was also partially impaired in Thbs1(-/-) mice. These data indicate that TSP-1 contributes to shear-dependent TGF-beta 1 activation, thus providing a potential explanation for the inconsistent in vitro data previously reported as well as for the differences in phenotypes of Thbs1(-/-) and Tgfb1(-/-) mice.
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