Journal
PLOS ONE
Volume 3, Issue 8, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0002966
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Funding
- Juvenile Diabetes Research Foundation [postdoctoral fellow]
- National Institute for Health Research (NIHR)
- Cambridge Biomedical Research Centre
- Wellcome Trust [079895]
- British Heart Foundation [FS/05/061/19501]
- Scientifical and Technical Research Council of Turkey [TUBITAK-SBAG3334]
- International Centre for Genetic Engineering and Biotechnology [ICGEB-CRP/TUR04-01]
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Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays.
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