Journal
PLOS ONE
Volume 3, Issue 3, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0001859
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Funding
- Fondation Medic
- Swiss National Science Foundation
- Canton de Geneve
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Background: The estrogen receptor alpha (ER alpha) is a ligand-regulated transcription factor. However, a wide variety of other extracellular signals can activate ER alpha in the absence of estrogen. The impact of these alternate modes of activation on gene expression profiles has not been characterized. Methodology/Principal Findings: We show that estrogen, growth factors and cAMP elicit surprisingly distinct ER alpha-dependent transcriptional responses in human MCF7 breast cancer cells. In response to growth factors and cAMP, ER alpha primarily activates and represses genes, respectively. The combined treatments with the anti-estrogen tamoxifen and cAMP or growth factors regulate yet other sets of genes. In many cases, tamoxifen is perverted to an agonist, potentially mimicking what is happening in certain tamoxifen-resistant breast tumors and emphasizing the importance of the cellular signaling environment. Using a computational analysis, we predicted that a Hox protein might be involved in mediating such combinatorial effects, and then confirmed it experimentally. Although both tamoxifen and cAMP block the proliferation of MCF7 cells, their combined application stimulates it, and this can be blocked with a dominant-negative Hox mutant. Conclusions/Significance: The activating signal dictates both target gene selection and regulation by ER alpha, and this has consequences on global gene expression patterns that may be relevant to understanding the progression of ER alpha-dependent carcinomas.
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