Journal
PLATELETS
Volume 20, Issue 6, Pages 391-400Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/09537100903114537
Keywords
MicroRNA; megakaryocytes; laser microdissection; primary myelofibrosis; essential thrombocythemia
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Funding
- Hochschul-interne Leistungsforderung - HiLF [11/07]
- Medizinische Hochschule Hannover
- Deutsche Krebshilfe
- Dr. Mildred Scheel Stiftung [10-2191]
- Deutsche Forschungsgemeinschaft - DFG [BO 1954/1-1]
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In primary myelofibrosis (PMF) and essential thrombocythemia (ET) the megakaryocytic lineage characteristically shows aberrant proliferation and maturation in which the regulatory microRNA (miR) system might be involved. Laser-microdissected PMF and ET megakaryocytes were analysed with real-time polymerase chain reaction (PCR) low density arrays comprising 365 microRNAs. The highest megakaryocytic expression levels were observed for miR-223, which is known to be expressed also in granulopoiesis. Cluster analysis revealed a tendency of disease-specific megakaryocytic microRNA expression profiles indicating that a complex shift of microRNA expression appears to be the underlying defect. Increased accumulation of miR-146b was observed in cellular stage PMF (p = 0.0125) but not ET megakaryopoiesis. In conclusion, this is the first microRNA profiling of in situ-derived PMF, ET and normal megakaryocytes.
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