Journal
PLASMID
Volume 65, Issue 3, Pages 226-231Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2011.02.003
Keywords
Indigenous plasmids; Horizontal mobility; Conjugation; Catabolic plasmids; Phosphotriesterase
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Funding
- CSIR, New Delhi
- SRF from ICMR, New Delhi
- DST
- DBT
- CSIR
- DBT-CREBB
- UGC-CAS
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A fosmid library of the 66 kb indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonas diminuta was tagged with mini-transposon EZTn5 < R6K gamma ori/KAN-2 >, to determine its sequence using transposon-specific primers. The sequence revealed the presence of a number of tra genes suggesting their role in conjugal transfer of pCMS1. Consistent with the presence of the tra genes, the B. diminuta plasmid, pCMS1::tet, generated by replacing the opd gene with opd::tet, served as a donor for transferring pCMS1::tet into recipient strain Pseudomonas putida. The self-transmissibility of the opd-containing plasmid pCMS1 and the existence of identical opd genes on otherwise dissimilar plasmids suggests a probable role of indigenous opd plasmids like pCMS1 in transferring the opd gene among soil bacteria.
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