Journal
PLASMA PROCESSES AND POLYMERS
Volume 9, Issue 1, Pages 59-66Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppap.201100031
Keywords
atmospheric-pressure plasmas; cancer cell treatment; cell death; intracellular effects; reactive species
Funding
- National Natural Science Foundation [10875048, 51077063]
- Ministry of Education, People's Republic of China
- Genetically Modified New Varieties of Plant and Animal of Major Projects of China [2009ZX08016-001A]
- Ministry of Science and Technology, People's Republic of China [2009DFB20290]
- State Bureau of Oceanic Administration, People's Republic of China [201005013]
- CSIRO
- Australian Research Council
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Reports show that cold atmospheric-pressure plasmas can induce death of cancer cells in several minutes. However, very little is presently known about the mechanism of the plasma-induced death of cancer cells. In this paper, an atmospheric-pressure plasma plume is used to treat HepG2 cells. The experimental results show that the plasma can effectively control the intracellular concentrations of ROS, NO and lipid peroxide. It is shown that these concentrations are directly related to the mechanism of the HepG2 death, which involves several stages. First, the plasma generates NO species, which increases the NO concentration in the extracellular medium. Second, the intracellular NO concentration is increased due to the NO diffusion from the medium. Third, an increase in the intracellular NO concentration leads to the increase of the intracellular ROS concentration. Fourth, the increased oxidative stress results in more effective lipid peroxidation and consequently, cell injury. The combined action of NO, ROS and lipid peroxide species eventually results in the HepG2 cell death.
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