Journal
PLANTA MEDICA
Volume 77, Issue 13, Pages 1528-1530Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-0030-1270834
Keywords
matrix metalloproteinase (MMP)-13; Cyathula officinalis Kuan; Amaranthacea; cartilage degradation; atractylenolide I; 1,3-diacetoxy-tetradeca-6E,12E-dien-8,10-dyn
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Funding
- National Research Foundation [2009-0071749]
- Ministry of Education, Science, and Technology (Korea)
- National Research Foundation of Korea [2009-0071749] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The roots of Cyathula officinalis Kuan are widely used in Chinese medicine for the treatment of inflammatory disorders. Here, the ability of C. officinalis Kuan to downregulate matrix metalloproteinase (MMP)-13 was examined since MMP-13 is an important enzyme for the degradation of the cartilage collagen matrix, especially under arthritic conditions. The ethanol extract of C. officinalis Kuan as well as the n-hexane and chloroform soluble fractions were found to potently inhibit MMP-13 induction in IL-1 beta-treated SW1353 cells, a human chondrosarcoma cell line, at 50200 mu g/mL. Activity-guided separation led to the isolation of six compounds, palmitic acid (1), beta-sitosterol (2), alpha-spinasterol (3), atractylenolide I (4), 1,3-diacetoxy-tetradeca-6E, 12E-dien-8,10-dyn (5), and N-trans-feruloyl-3-methyldopamine (6). Among these, 4 and 5 exhibited MMP-13 downregulating activity in IL-1 beta-treated SW1353 cells. And 4 also showed anti-oedematous activity against lambda-carageenan-induced paw edema in mice at 20-200 mg/kg, p.o. The results of this study provide information that can help elucidate the action mechanism of C. officinalis Kuan. In addition, the results presented here suggest that C. officinalis Kuan and its constituents may have the potential for chondroprotection against cartilage degrading disorders.
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