Journal
PLANTA MEDICA
Volume 77, Issue 1, Pages 40-45Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-0030-1250121
Keywords
7-epiclusianone; Rheedia brasiliensis; Guttiferae; Streptococcus mutans; GtfB; biofilm; dental caries
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Funding
- FAPESP (State of Sao Paulo Research Foundation, Brazil) [06/56379-4]
- CNPq (National Council for Scientific and Technological Development, Brazil) [131751/2006-9, 141253/2005-3]
- CAPES (PDEE/CAPES, Brazil) [BEX 0154/06-7]
- National Institute of Dental and Craniofacial Research (National Institutes of Health) [1R01 DE018023]
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE018023] Funding Source: NIH RePORTER
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The aim of this study was to evaluate the effects of 7-epiclusianone (7-epi) on specific virulence attributes of Streptococcus mutans in vitro and on development of dental caries in vivo. 7-Epi was obtained and purified from fruits of Rheedia brasiliensis. We investigated its influence on surface-adsorbed glucosyltransferase (Gtf) B activity, acid production, and viability of S. mutans in biofilms, as well as on caries development using a rodent model. 7-Epi (100 mu g/mL) significantly reduced the activity of surface-adsorbed GtfB (up to 48.0 +/- 1.8 of inhibition at 100 mu g/mL) and glyco-lytic pH-drop by S. mutans in biofilms (125 and 250 mu g/mL) (vs. vehicle control, p < 0.05). In contrast, the test compound did not significantly affect the bacterial viability when compared to vehicle control (15% ethanol, p > 0.05). Wistar rats treated topically with 7-epi (twice daily, 60-s exposure) showed significantly smaller number of and less severe smooth-and sulcal-surface carious lesions (p < 0.05), without reducing the S. mutans viable population from the animals' dental biofilms. In conclusion, the natural compound 7-epiclusianone may be a potentially novel pharmacological agent to prevent and control dental caries disease.
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